The PLCZ1 Polyclonal Antibody (PAC016875) is a valuable tool for researchers studying PLCZ1, a key protein involved in sperm activation and fertilization. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.PLCZ1 is a crucial player in the process of egg activation during fertilization, as it is responsible for triggering the calcium oscillations necessary for embryo development. By targeting PLCZ1 with this antibody, researchers can effectively study and analyze the protein's function and expression levels in various cell types.
The importance of PLCZ1 in reproductive biology and fertility research cannot be overstated, as understanding its role in sperm activation can lead to advancements in assisted reproductive technologies and potential treatments for infertility. The PLCZ1 Polyclonal Antibody is a valuable tool for investigating the mechanisms underlying fertilization and may provide insights into improving reproductive outcomes.
Antibody Name:
PLCZ1 Antibody (PACO16875)
Antibody SKU:
PACO16875
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:2000, IHC:1:25-1:100
Species Reactivity:
Human
Immunogen:
Fusion protein of human PLCZ1
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO16875(PLCZ1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants.
Synonyms:
phospholipase C, zeta 1
UniProt Protein Function:
PLCZ1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; PhospholipaseChromosomal Location of Human Ortholog: 12p12.3Cellular Component: perinuclear region of cytoplasm; nucleus; cytosolMolecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activityBiological Process: inositol phosphate metabolic process; multicellular organismal development; calcium ion transport; egg activation; lipid catabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
