The PLCZ1 Polyclonal Antibody (PAC037258) is a valuable tool for researchers studying PLCZ1, a crucial enzyme involved in egg activation during fertilization. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot experiments. By binding to the PLCZ1 protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential asset for studies in reproductive biology and fertility research.PLCZ1, also known as phospholipase C zeta 1, plays a pivotal role in the process of sperm-induced egg activation, triggering the events necessary for successful fertilization.
Its involvement in this key reproductive event makes PLCZ1 a promising target for investigating infertility issues and developing assisted reproductive technologies. Understanding the function of PLCZ1 is crucial for advancing our knowledge of reproductive biology and potentially improving fertility treatments for couples struggling with conception.
Antibody Name:
PLCZ1 Antibody (PACO37258)
Antibody SKU:
PACO37258
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 protein (1-415AA)
Western blot. All lanes: PLCZ1 antibody at 3µg/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse kidney tissue. Lane 3: Human placenta tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 71, 48, 58 kDa. Observed band size: 71 kDa.
Background:
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
PLCZ1: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; PhospholipaseChromosomal Location of Human Ortholog: 12p12.3Cellular Component: perinuclear region of cytoplasm; nucleus; cytosolMolecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activityBiological Process: inositol phosphate metabolic process; multicellular organismal development; calcium ion transport; egg activation; lipid catabolic process
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
