The Plekha8 Polyclonal Antibody (PAC040150) is a vital tool for researching Plekha8, a protein involved in cell adhesion and signaling pathways. This antibody, produced using rabbits, shows strong reactivity with human samples and is validated for use in various applications, including Western blotting. By binding to the Plekha8 protein, this antibody allows for precise detection and analysis in different cell types, making it particularly useful for studies in cell biology and signal transduction.Plekha8, also known as Pleckstrin homology domain-containing family A member 8, plays a crucial role in cell-cell adhesion and communication, making it a key player in processes like tissue development and maintenance.
Understanding the functions and regulatory mechanisms of Plekha8 can provide valuable insights into various physiological and pathological processes, including cancer metastasis and tissue regeneration. Utilizing this antibody to explore Plekha8 can lead to significant advancements in our understanding of cellular behavior and disease mechanisms.
Western blot. All lanes: PLEKHA8 antibody at 0.8µg/ml + Mouse brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 59, 52, 50 kDa. Observed band size: 59 kDa.
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO40150 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human placenta tissue using PACO40150 at dilution of 1:100.
Background:
Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation.
Synonyms:
Pleckstrin homology domain-containing family A member 8 (PH domain-containing family A member 8) (Phosphatidylinositol-four-phosphate adapter protein 2) (FAPP-2) (Phosphoinositol 4-phosphate adapter protein 2) (hFAPP2) (Serologically defined breast cancer antigen NY-BR-86), PLEKHA8, FAPP2
UniProt Protein Function:
PLEKHA8: Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans- Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 7p14.3Cellular Component: trans-Golgi networkMolecular Function: glycolipid binding; glycolipid transporter activityBiological Process: lipid transport
Pleckstrin homology domain-containing family A member 8
NCBI Synonym Full Names:
pleckstrin homology domain containing A8
NCBI Official Symbol:
PLEKHA8Â Â
NCBI Official Synonym Symbols:
FAPP2Â Â
NCBI Protein Information:
pleckstrin homology domain-containing family A member 8
UniProt Protein Name:
Pleckstrin homology domain-containing family A member 8
UniProt Synonym Protein Names:
Phosphatidylinositol-four-phosphate adapter protein 2; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2; Serologically defined breast cancer antigen NY-BR-86
