The PLZF Monoclonal Antibody (CAB21185) is a high-quality antibody developed for reliable detection and analysis of target proteins. PLZF is a transcription factor that plays a key role in regulating gene expression and is implicated in various biological processes, including cell differentiation and development.This monoclonal antibody, produced using hybridoma technology, is highly specific for PLZF and has been validated for use in immunohistochemistry, flow cytometry, and other experimental techniques. Its ability to target PLZF enables precise detection and analysis of this important protein in a variety of cell types and tissues, making it an invaluable tool for researchers in the fields of stem cell biology, oncology, and developmental biology.
This antibody is validated for use in WB, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PLZF Monoclonal Antibody
SKU:
CAB21185
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC52265
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
2μg-4μg antibody for 400μg-1000μg extracts of whole cells
IF-P
1:200 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PLZF, ZNF145
Positive Sample:
293F, Mouse heart, Rat brain, Mouse heart
Cellular Localization:
Nucleus.
Calculated MW:
74kDa
Observed MW:
80kDa/80kd
This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.
Purification Method
Affinity purification
Gene ID
7704
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PLZF Rabbit mAb (CAB21185) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Mouse heart, using PLZF Rabbit mAb (CAB21185) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Confocal imaging of paraffin-embedded Rat testis tissue using PLZF Rabbit mAb (CAB21185, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunoprecipitation of PLZF from 1000 µg extracts of Mouse heart was performed using 2 µg of PLZF Rabbit mAb (CAB21185). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using PLZF Rabbit mAb (CAB21185) at a dilution of 1:15000.
