The PNCK Polyclonal Antibody (PAC061029) is a valuable tool for researchers studying PNCK, a protein involved in various cellular processes, including cell signaling and gene regulation. This antibody, produced in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications. By targeting the PNCK protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential reagent for studies in molecular biology and cancer research.
PNCK, also known as a serine-threonine protein kinase, plays a crucial role in regulating cell growth, proliferation, and differentiation. Its involvement in key cellular pathways makes it a promising target for investigating diseases such as cancer and neurodegenerative disorders. By understanding the function of PNCK, researchers can uncover potential therapeutic strategies for targeting this protein in disease treatments.
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse brain tissue, Mouse kidney tissue. All lanes: PNCK antibody at 3.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39, 41, 28, 47 kDa. Observed band size: 39 kDa.
IHC image of PACO61029 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).
Synonyms:
Calcium/calmodulin-dependent protein kinase type 1B (EC 2.7.11.17) (CaM kinase I beta) (CaM kinase IB) (CaM-KI beta) (CaMKI-beta) (Pregnancy up-regulated non-ubiquitously-expressed CaM kinase), PNCK
UniProt Protein Function:
CAMK1B: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family
Chromosomal Location of Human Ortholog: Xq28
NCBI Summary:
PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008]
