The PNPLA3 Antibody (CAB6694) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit serum, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PNPLA3 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for studies in metabolic disorders and liver diseases.PNPLA3, also known as adiponutrin, is a lipase enzyme that plays a critical role in lipid droplet metabolism, triglyceride storage, and energy homeostasis. Mutations in the PNPLA3 gene have been associated with liver conditions such as non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH), making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PNPLA3 Antibody
SKU:
CAB6694
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ADPN, C22orf20, iPLA(2)epsilon, PNPLA3
Positive Sample:
SKOV3, Mouse lung, Rat liver
Cellular Localization:
Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
53kDa
Observed MW:
53kDa
The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes.
Purification Method
Affinity purification
Gene ID
80339
RRID
AB_2767278
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PNPLA3 Rabbit pAb (CAB6694) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
