The POLL Antibody (CAB8408) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot applications. By specifically binding to POLL, this antibody allows for precise detection and analysis of the protein in various cell types, making it an excellent choice for studies in DNA repair mechanisms and cancer research.POLL, also known as DNA polymerase lambda, is essential for error-prone DNA repair pathways and plays a crucial role in maintaining genomic integrity.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POLL Antibody
SKU:
CAB8408
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BETAN, POLKAPPA, POLL
Positive Sample:
MCF7, HL-60, A-549, NCI-H460, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
63kDa
Observed MW:
63kDa
This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
27343
RRID
AB_2771739
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POLL Rabbit pAb (CAB8408) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
