The POLR2C Antibody (CAB1785) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POLR2C Antibody
SKU:
CAB1785
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPB3, RPB31, hRPB33, hsRPB3, RPB3/POLR2C
Positive Sample:
SW620, OVCAR3, Mouse spleen
Cellular Localization:
Nucleus.
Calculated MW:
31kDa
Observed MW:
38kDa
This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.
Purification Method
Affinity purification
Gene ID
5432
RRID
AB_2763827
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RPB3/POLR2C Rabbit pAb (CAB1785) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 150 μg extracts of SW620 cells using 3 μg RPB3/POLR2C antibody (CAB1785). Western blot was performed from the immunoprecipitate using RPB3/POLR2C antibody (CAB1785) at a dilution of 1:500.
