The POLR2F Antibody (CAB6837) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the POLR2F protein, it allows for the detection and analysis of POLR2F in a variety of cell types, making it ideal for studies in molecular biology and gene regulation.POLR2F plays a crucial role in the transcription process, serving as a key component of the RNA polymerase II complex responsible for transcribing protein-coding genes. Understanding the function of POLR2F is essential for deciphering the mechanisms of gene expression and regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
POLR2F Antibody
SKU:
CAB6837
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5435
RRID
AB_2767404
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat heart, using POLR2F Rabbit pAb (CAB6837) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
