The POLR2J Antibody (CAB1843) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the POLR2J protein, it allows for the detection and analysis of this crucial transcription factor in a variety of cell types.The POLR2J protein is involved in the transcription of protein-coding genes and is crucial for the regulation of gene expression. Understanding its function and activity is essential for unraveling the complex mechanisms of transcription and gene regulation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POLR2J Antibody
SKU:
CAB1843
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPB11, RPB11A, RPB11m, hRPB14, POLR2J1, POLR2J
Positive Sample:
K-562, Mouse thymus
Cellular Localization:
Nucleus.
Calculated MW:
13kDa
Observed MW:
16kDa
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
Purification Method
Affinity purification
Gene ID
5439
RRID
AB_2763879
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POLR2J Rabbit pAb (CAB1843) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using POLR2J Rabbit pAb (CAB1843). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
