The POLR3E Polyclonal Antibody (PACO06772) is a valuable tool for research involving POLR3E, a subunit of RNA polymerase III essential for transcription. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot and immunohistochemistry applications. By targeting the POLR3E protein, researchers can detect and analyze its expression in various cell types, offering insights into transcriptional regulation and potential implications for diseases such as cancer and genetic disorders.
POLR3E is a crucial component of the RNA polymerase III complex, responsible for transcribing small structural RNAs essential for cellular function. Its role in gene expression and regulation makes it an attractive target for investigating transcriptional dysregulation in disease states. By gaining a better understanding of POLR3E dynamics, researchers can uncover novel therapeutic strategies for targeting transcriptional abnormalities in cancer and other disorders.
Synthesized peptide derived from the Internal region of human POLR3E.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of Jurkat K562 cells using POLR3E Polyclonal Antibody.
Western Blot analysis of JK cells using POLR3E Polyclonal Antibody.
Synonyms:
POLR3E; KIAA1452; DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; DNA-directed RNA polymerase III 80 kDa polypeptide
UniProt Protein Function:
POLR3E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription initiation complex; EC 2.7.7.6; TransferaseChromosomal Location of Human Ortholog: 16p12.2Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasmBiological Process: defense response to virus; gene expression; innate immune response; positive regulation of interferon type I production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter
