The POLR3E Polyclonal Antibody (PAC040882) from Assay Genie is a valuable tool for researchers studying POLR3E, a subunit of RNA polymerase III involved in transcription. This antibody is produced in rabbits and is highly specific for human samples, making it an ideal choice for Western blot applications.POLR3E plays a crucial role in various biological processes, including cell growth and development, and aberrant expression has been linked to diseases such as cancer and neurodevelopmental disorders. By using this antibody, researchers can reliably detect and analyze POLR3E protein levels in different cell types, shedding light on its functions and potential therapeutic implications.
With its high reactivity and specificity, the POLR3E Polyclonal Antibody (PAC040882) is a valuable tool for scientists working in the fields of molecular biology, genetics, and biochemistry. By elucidating the role of POLR3E in cellular processes, this antibody holds promise for advancing our understanding of disease mechanisms and exploring new avenues for drug development.
Antibody Name:
POLR3E Antibody (PACO40882)
Antibody SKU:
PACO40882
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human DNA-directed RNA polymerase III subunit RPC5 protein (1-260AA)
Immunohistochemistry of paraffin-embedded human testis tissue using PACO40882 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human skin tissue using PACO40882 at dilution of 1:100.
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).
Synonyms:
DNA-directed RNA polymerase III subunit RPC5 (RNA polymerase III subunit C5) (DNA-directed RNA polymerase III 80 kDa polypeptide), POLR3E, KIAA1452
UniProt Protein Function:
POLR3E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription initiation complex; EC 2.7.7.6; TransferaseChromosomal Location of Human Ortholog: 16p12.2Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasmBiological Process: defense response to virus; gene expression; innate immune response; positive regulation of interferon type I production; RNA elongation from RNA polymerase III promoter; termination of RNA polymerase III transcription; transcription from RNA polymerase III promoter
