The POLR3K Antibody (CAB6467) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. It specifically binds to the POLR3K protein, allowing for the detection and analysis of POLR3K expression in various cell types.POLR3K is a key component of the RNA polymerase III complex, essential for the transcription of small RNAs. Dysregulation of POLR3K has been linked to various diseases, including cancer and neurodevelopmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
POLR3K Antibody
SKU:
CAB6467
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.
Purification Method
Affinity purification
Gene ID
51728
RRID
AB_2767069
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POLR3K Rabbit pAb (CAB6467) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
