The POP4 Antibody (CAB12563) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By targeting the POP4 protein, researchers can effectively detect and analyze its expression in various cell types, making it an essential tool for investigations in molecular biology and gene regulation.POP4, a component of the RNase P complex, plays a crucial role in cleaving precursor tRNAs to generate mature tRNAs, essential for protein synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
POP4 Antibody
SKU:
CAB12563
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPP29, POP4
Positive Sample:
MCF7, Rat spleen
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
25kDa
Observed MW:
26kDa
This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10775
RRID
AB_2759405
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POP4 Rabbit pAb (CAB12563) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
