The POP4 Antibody (CAB4452) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POP4 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential asset for studies in molecular biology and genetics.POP4 is an essential component of the RNA processing machinery, involved in cleavage and polyadenylation of mRNA transcripts. Its role in gene expression regulation makes it a crucial target for research into various biological processes, including RNA metabolism, protein synthesis, and cellular differentiation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POP4 Antibody
SKU:
CAB4452
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPP29, POP4
Positive Sample:
SH-SY5Y, A-549, HL-60
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
25kDa
Observed MW:
25kDa
This gene encodes one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is localized to the nucleus and associates directly with the RNA component of these complexes. This protein is involved in processing of precursor RNAs. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10775
RRID
AB_2765686
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POP4 Rabbit pAb (CAB4452) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using POP4 Rabbit pAb (CAB4452) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using POP4 Rabbit pAb (CAB4452) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
