The POP5 Antibody (CAB16530) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards POP5 in human samples, making it suitable for Western blot applications. By recognizing and binding to the POP5 protein, this antibody enables the accurate detection and analysis of POP5 expression in various cell types.POP5 is a crucial component of the RNase MRP complex, which plays a vital role in the cleavage of RNA molecules and the maturation of ribosomal RNA.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
POP5 Antibody
SKU:
CAB16530
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPP2, RPP20, hPop5, HSPC004, POP5
Positive Sample:
A-549, K562, 293T
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
19kDa
Observed MW:
19kDa
Enables ribonuclease P RNA binding activity. Contributes to ribonuclease P activity. Involved in tRNA 5'-leader removal. Located in nucleolus. Part of multimeric ribonuclease P complex and ribonuclease MRP complex.
Purification Method
Affinity purification
Gene ID
51367
RRID
AB_2771750
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POP5 Rabbit pAb (CAB16530) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
