Porcine cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
The Porcine cTnT/TNNT2 (Cardiac Troponin T Type 2) ELISA Kit is a specialized assay for the quantitative detection of cTnT, a cardiac muscle protein, in porcine samples. Cardiac troponin T is a key biomarker in cardiotoxicity studies, myocardial infarction, and other heart-related conditions. By measuring cTnT levels accurately, researchers can gain insights into cardiac injury, assess cardiac function, and monitor heart health in porcine models.
Our Porcine cTnT/TNNT2 ELISA Kit from Assay Genie offers high sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality controls, this kit guarantees robust performance and ease of use, making it an excellent tool for porcine cardiac research focused on troponin T analysis.
Product Name:
Porcine cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
SKU:
AEES00280
Target:
Porcine cTnT/TNNT2 (Troponin T Type 2, Cardiac)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine cTnT/TNNT2. You can calculate the concentration of Porcine cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
87-102
96-109
Average (%)
106
93
102
1:4
Range (%)
98-111
84-96
94-110
Average (%)
104
90
102
1:8
Range (%)
95-108
88-104
97-112
Average (%)
101
95
103
1:16
Range (%)
100-115
86-101
95-110
Average (%)
107
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.99
104.98
435.55
52.56
100.2
444.7
Standard deviation
3.09
4.49
18.38
3.52
5.77
19.57
C V (%)
6.18
4.28
4.22
6.7
5.76
4.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine cTnT/TNNT2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Porcine cTnT/TNNT2 and analogues was observed.
