Porcine Glutathione S Transferase Pi (GSTp) ELISA Kit
The Porcine Glutathione S-Transferase Pi (GSTP) ELISA Kit is a reliable and accurate tool for the quantitative measurement of GSTP levels in porcine samples. This kit is designed for use with porcine serum, plasma, and tissue lysates, offering high sensitivity and specificity for consistent and reproducible results.GSTP is a key enzyme involved in the detoxification of xenobiotics and oxidative stress, playing a critical role in protecting cells from damage. Dysregulation of GSTP has been implicated in various diseases, including cancer, liver diseases, and neurodegenerative disorders, making it a valuable biomarker for research and diagnostic purposes.
With its user-friendly protocol and quick assay time, the Porcine GSTP ELISA Kit is suitable for a wide range of research applications, providing valuable insights into the role of GSTP in porcine health and disease. Trust AssayGenie for reliable and accurate results with this high-quality ELISA kit.
Product Name:
Porcine Glutathione S Transferase Pi (GSTp) ELISA Kit
Product Code:
PRDL00063
Size:
96 Assays
Target:
GSTp
Synonyms:
GSTP1, GST-P1, DFN7, FAEES3, GST3
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
