The Porcine IL-12 ELISA Kit is a cutting-edge assay designed for the precise and quantitative measurement of Interleukin-12 (IL-12) levels in porcine biological samples. IL-12 is a crucial cytokine involved in modulating immune responses, particularly Th1 cell differentiation and the production of pro-inflammatory mediators. Its role in regulating innate and adaptive immunity makes IL-12 a key mediator in infectious diseases, autoimmune conditions, and immunodeficiency disorders. This ELISA kit empowers researchers to quantify IL-12 concentrations in porcine samples, providing valuable insights into the immune response mechanisms and inflammatory processes in pigs.
By accurately measuring IL-12 levels, researchers can explore the role of this cytokine in immune regulation, host defense mechanisms, and disease pathogenesis in porcine models. With outstanding sensitivity and specificity, the Porcine IL-12 ELISA Kit ensures accurate and reproducible results, enabling researchers to investigate the impact of IL-12 on immune modulation and inflammatory responses in pigs. Manufactured under stringent quality control standards, this kit offers robust performance and simplicity of use, making it the ideal choice for porcine immunology research and studies on immune-mediated diseases.
Product Name:
Porcine IL-12 ELISA Kit
SKU:
AEES00720
Target:
Porcine IL-12
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine IL-12. You can calculate the concentration of Porcine IL-12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
91-107
93-105
Average (%)
96
99
98
1:4
Range (%)
85-97
94-107
91-105
Average (%)
91
101
96
1:8
Range (%)
88-103
89-100
91-103
Average (%)
94
94
98
1:16
Range (%)
88-98
92-109
89-100
Average (%)
93
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.1
256.86
758.5
93.26
254.09
775.53
Standard deviation
6.08
13.0
31.78
4.87
13.06
36.99
C V (%)
5.9
5.06
4.19
5.22
5.14
4.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine IL-12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine IL-12 in samples. No significant cross-reactivity or interference between Porcine IL-12 and analogues was observed.
