Porcine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit
Based on the information provided in the URL, I have developed the following product description:The Porcine Tissue Inhibitors of Metalloproteinase-3 (TIMP3) ELISA Kit is a reliable and accurate tool for the measurement of TIMP3 levels in porcine tissue samples. TIMP3 is a key regulator of metalloproteinase activity, playing a critical role in tissue remodeling and extracellular matrix turnover.This ELISA kit offers high sensitivity and specificity, allowing for precise quantification of TIMP3 levels in a variety of porcine biological samples. With its reproducible results, this kit is suitable for a wide range of research applications, including studies on inflammatory diseases, tissue fibrosis, and wound healing.
TIMP3 dysregulation has been implicated in various pathologies, including cardiovascular diseases and cancer progression. By accurately measuring TIMP3 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. Overall, the Porcine TIMP3 ELISA Kit provides a valuable tool for researchers studying TIMP3 biology and its role in disease pathology.
Product Name:
Porcine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
