The PORCN Antibody (CAB12250) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the PORCN protein, this antibody enables accurate detection and analysis in a variety of cell types, making it well-suited for studies in developmental biology, cancer research, and regenerative medicine.PORCN is a critical component of the Wnt signaling pathway, which plays a key role in various cellular processes such as cell proliferation, differentiation, and migration. Dysregulation of this pathway has been implicated in a wide range of diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PORCN Antibody
SKU:
CAB12250
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method
Affinity purification
Gene ID
64840
RRID
AB_2759121
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PORCN Rabbit pAb (CAB12250) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
