The POT1 Antibody (CAB1491) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the POT1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for investigations in the fields of genetics and cancer research.POT1, also known as protection of telomeres 1, plays a vital role in protecting chromosome ends and regulating telomere length, which is essential for maintaining genomic integrity and preventing cellular aging.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POT1 Antibody
SKU:
CAB1491
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GLM9, CMM10, HPOT1, POT1
Positive Sample:
A-431, HeLa, DU145, Mouse liver
Cellular Localization:
Chromosome, Nucleus, Telomere.
Calculated MW:
71kDa
Observed MW:
75kDa
This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
25913
RRID
AB_2761791
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POT1 Rabbit pAb (CAB1491) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
