The POU2AF1 Antibody (CAB6696) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the POU2AF1 protein, allowing for accurate detection and analysis in various cell types.POU2AF1, also known as OBF-1, plays a crucial role in the regulation of genes involved in B cell differentiation and activation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POU2AF1 Antibody
SKU:
CAB6696
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BOB1, OBF1, OCAB, OBF-1, POU2AF1
Positive Sample:
Raji, Daudi
Cellular Localization:
Nucleus.
Calculated MW:
27kDa
Observed MW:
35kDa
Enables transcription coactivator activity. Involved in positive regulation of transcription by RNA polymerase II. Part of RNA polymerase II transcription regulator complex.
Purification Method
Affinity purification
Gene ID
5450
RRID
AB_2767280
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using POU2AF1 Rabbit pAb (CAB6696) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
