The POU4F3 Antibody (CAB7712) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. RRID AB_2771753 Gene ID 5459 Swiss Prot Synonym BRN3C; DFNA15; DFNA42; DFNA52; POU4F3
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
POU4F3 Antibody
SKU:
CAB7712
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRN3C, DFNA15, DFNA42, DFNA52, POU4F3
Positive Sample:
Jurkat
Cellular Localization:
Nucleus.
Calculated MW:
37kDa
Observed MW:
37kDa
This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. RRID AB_2771753 Gene ID 5459 Swiss Prot Synonym BRN3C; DFNA15; DFNA42; DFNA52; POU4F3
Purification Method:
Affinity purification
Gene ID:
5459
RRID:
AB_2771753
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using POU4F3 Rabbit pAb (CAB7712) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
