The PPM1A Monoclonal Antibody (CAB22076) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mouse, is highly specific to human samples and has been validated for use in applications such as Western blot and immunofluorescence.PPM1A, also known as protein phosphatase Mg2+/Mn2+ dependent 1A, plays a crucial role in dephosphorylating target proteins, thereby regulating their function and activity. Dysregulation of PPM1A has been implicated in various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PPM1A Monoclonal Antibody
SKU:
CAB22076
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC55164
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
Purification Method
Affinity purification
Gene ID
5494
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPM1A Rabbit mAb (CAB22076) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
