Description
PPP1R12B Antibody (CAB17510)
The PPP1R12B Antibody (CAB17510) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human samples and is validated for use in Western blot applications. By binding to PPP1R12B, researchers can detect and analyze the protein in various cell types, making it ideal for studies in cell biology and signal transduction pathways.PPP1R12B is known to play a crucial role in controlling actin cytoskeleton dynamics and cell migration, making it a target of interest in cancer research and other diseases involving cell motility.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | PPP1R12B Antibody |
| SKU: | CAB17510 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | TFSR SRAE RQAQ EQPR EKPT DTEG LEGS PEKH EPSA VPAT EAGE GQQP WGRS LDEE PICH RLRC PAQP DKPT TPAS PSTS RPSL YTSS HLLW TNRF SVPD SESS ETTT NTTT AKEM DKNE NEEA DLDE QSSK RLSI RERR RPKE RRRG TGIN FWTK DEDE TDGS EEVK ETWH ERLS RLES GGSN PTTS DSYG DRAS ARAR REAR EARL ATLT SRVE EDSN RDYK KLYE SALT ENQK LKTK LQEA QLEL ADIK SKLE KVAQ QKQE KTSD RSS | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | MYPT2, PP1bp55, PPP1R12B |
| Positive Sample: | 293T, U-87MG |
| Cellular Localization: | A Band, Cytoplasm, Cytoskeleton, Cytosol, Nucleoplasm, Z Disc. |
| Calculated MW: | 110kDa |
| Observed MW: | 110kDa |
Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome.
| Purification Method | Affinity purification |
| Gene ID | 4660 |
| RRID | AB_2771770 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using PPP1R12B Rabbit pAb (CAB17510) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
