The PPP1R14A Antibody (CAB10160) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications, allowing for accurate detection and analysis of PPP1R14A protein levels in human samples.PPP1R14A, also known as protein phosphatase 1 regulatory subunit 14A, plays a crucial role in regulating smooth muscle contraction and cell motility. Dysfunction of PPP1R14A has been linked to various diseases, including cancer and cardiovascular disorders. By targeting PPP1R14A, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify novel therapeutic targets.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PPP1R14A Antibody
SKU:
CAB10160
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CPI17, CPI-17, PPP1INL, PPP1R14A
Positive Sample:
K-562, Mouse lung, Mouse brain, Mouse spleen, Rat lung, Rat testis
Cellular Localization:
Cytoplasm.
Calculated MW:
17kDa
Observed MW:
19kDa
The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
94274
RRID
AB_2757688
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPP1R14A Rabbit pAb (CAB10160) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
