The PPP1R14C Polyclonal Antibody (PAC021902) is a valuable tool for researchers studying PPP1R14C, a protein involved in cellular processes such as muscle contraction and cell division. The antibody, produced in rabbits, displays high specificity towards human samples and is suitable for use in Western blot experiments. By binding to the PPP1R14C protein, this antibody enables the detection and analysis of PPP1R14C in various cell types, making it an essential component of studies in cell biology and molecular biology.PPP1R14C, also known as protein phosphatase 1 regulatory subunit 14C, plays a crucial role in regulating the activity of protein phosphatase 1 (PP1) in cells.
This function makes PPP1R14C a key player in processes like muscle relaxation and cell cycle progression. Research on PPP1R14C is important for understanding how cellular signaling pathways are controlled and how dysregulation of these pathways can contribute to diseases such as cancer and cardiovascular disorders. By investigating the functions of PPP1R14C, scientists can uncover new insights into the molecular mechanisms underlying these conditions and pave the way for the development of targeted therapeutic interventions.
Antibody Name:
PPP1R14C Antibody (PACO21902)
Antibody SKU:
PACO21902
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human PP14C.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells and COLO205 cells, using PP14C antibody.
Background:
Inhibitor of the PP1 regulatory subunit PPP1CA. Liu QR., J. Biol. Chem. 277:13312-13320(2002).
Synonyms:
Protein phosphatase 1 regulatory subunit 14C; PKCµpotentiated PP1 inhibitory protein; Kinase-enhanced PP1 inhibitor; Serologically defined breast cancer antigen NY-BR-81; PPP1R14C
UniProt Protein Function:
KEPI: Inhibitor of PPP1CA. Has over 600-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. Belongs to the PP1 inhibitor family.
UniProt Protein Details:
Protein type:Inhibitor; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 6q24.3-q25.3
Cellular Component: cytoplasm; membrane
Molecular Function:type 1 serine/threonine specific protein phosphatase inhibitor activity
Biological Process: negative regulation of catalytic activity; regulation of phosphorylation
NCBI Summary:
The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1 (Liu et al., 2002 [PubMed 11812771]).[supplied by OMIM, Feb 2010]
