The DARPP32 Antibody (CAB2580) is a high-quality antibody developed for reliable detection and analysis of target proteins. This protein plays a crucial role in the regulation of various cellular processes, including neurotransmission, neuronal signaling, and synaptic plasticity.The antibody, produced in rabbits, is highly specific to PPP1R1B and has been validated for use in Western blot and immunohistochemistry applications. It enables the detection and analysis of PPP1R1B in a variety of cell types and tissue samples, making it ideal for research in neurobiology, neuroscience, and psychiatry.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DARPP32 Antibody
SKU:
CAB2580
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DARPP32, DARPP-32
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
23kDa
Observed MW:
34kDa
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
84152
RRID
AB_2764466
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DARPP32 Rabbit pAb (CAB2580) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of SH-SY5Y cells using DARPP32 Rabbit pAb (CAB2580) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
