The PPP4R1 Antibody (CAB8361) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. RRID AB_2771789 Gene ID 9989 Swiss Prot Synonym MEG1; PP4R1; PP4(Rmeg); PPP4R1
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PPP4R1 Antibody
SKU:
CAB8361
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEG1, PP4R1, PP4(Rmeg), PPP4R1
Positive Sample:
293T, U-251MG, BT-474, K-562, SKOV3
Cellular Localization:
-
Calculated MW:
107kDa
Observed MW:
130kDa
This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. RRID AB_2771789 Gene ID 9989 Swiss Prot Synonym MEG1; PP4R1; PP4(Rmeg); PPP4R1
Purification Method:
Affinity purification
Gene ID:
9989
RRID:
AB_2771789
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPP4R1 Rabbit pAb (CAB8361) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg PPP4R1 antibody (CAB8361). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (CAB8361) at a dilution of 1:1000.
