The PPP4R1 Antibody (CAB8361) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PPP4R1 Antibody
SKU:
CAB8361
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEG1, PP4R1, PP4(Rmeg), PPP4R1
Positive Sample:
293T, U-251MG, BT-474, K-562, SKOV3
Calculated MW:
107kDa
Observed MW:
130kDa
This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
9989
RRID
AB_2771789
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PPP4R1 Rabbit pAb (CAB8361) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunoprecipitation analysis of 200 μg extracts of 293T cells, using 3 μg PPP4R1 antibody (CAB8361). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (CAB8361) at a dilution of 1:1000.
