The PRAME Antibody (CAB14507) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically targets the PRAME protein, allowing for accurate detection and analysis in a variety of cell types typically found in cancer research.PRAME, or Preferentially Expressed Antigen in Melanoma, is involved in promoting tumor growth and survival, making it a potential target for cancer therapy development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PRAME Antibody
SKU:
CAB14507
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAPE, OIP4, CT130, OIP-4, PRAME
Positive Sample:
U2OS, U-251MG, Mouse liver, Mouse lung, Rat lung
Cellular Localization:
Cell Membrane, Nucleus.
Calculated MW:
58kDa
Observed MW:
58kDa
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23532
RRID
AB_2761381
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRAME Rabbit pAb (CAB14507) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
