Protein A HCP ELISA Kit (HDES0123)

These kits are intended for use in quantitating recombinant and Alkali tolerant Protein A constructs such as MabSelect SuRe (Cytiva), MabSelect PrismA (Cytiva), MaXtar® ARPA ligand Protein A (Bio-Link), recombinant Protein A (TRUKING MICRO-SPPHERE). This kit is for Research Use Only and are not intended for diagnostic use in humans or animals. This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Protein A. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Protein A, HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Protein A. You can calculate the concentration of Protein A in the samples by comparing the OD of the samples to the standard curve.