The PRPS2 Antibody (CAB12645) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the PRPS2 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and cancer research.PRPS2 is a key enzyme in the purine biosynthesis pathway, playing a crucial role in maintaining cellular energy levels and nucleotide pool balance.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
PRPS2 Antibody
SKU:
CAB12645
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PRSII, PRPS2
Positive Sample:
HeLa, MCF7, Jurkat, Rat spinal cord
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
35kDa
Observed MW:
34kDa
This gene encodes a phosphoribosyl pyrophosphate synthetase that plays a central role in the synthesis of purines and pyrimidines. The encoded protein catalyzes the synthesis of 5-phosphoribosyl 1-pyrophosphate from ATP and D-ribose 5-phosphate. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5634
RRID
AB_2759490
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PRPS2 Rabbit pAb (CAB12645) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.