The Prune1 Polyclonal Antibody (PACO37362) is a valuable tool for researchers studying Prune1, a protein involved in cell proliferation and migration. This antibody, produced in rabbits, is highly specific to human samples and is widely used in Western blot applications. By binding to the Prune1 protein, this antibody enables the detection and analysis of Prune1 levels in various cell types, making it an essential component for studies in cancer biology and cell signaling pathways.Prune1, also known as phosphoesterase, plays a critical role in promoting cell growth and invasion, making it a key target for research in cancer progression and metastasis.
Understanding the function of Prune1 is essential for developing targeted therapies to inhibit its activity in cancer cells. By utilizing the Prune1 Polyclonal Antibody, researchers can further investigate the role of Prune1 in various cellular processes and potentially uncover new treatment strategies for cancer patients.
Western Blot. Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: PRUNE1 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 43, 31, 27, 25, 19 kDa. Observed band size: 60 kDa.
Immunofluorescence staining of HepG2 cells with PACO37362 at 1:135, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO37362 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.
PRUNE: Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. Belongs to the PPase class C family. Prune subfamily. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.6.1.1; Nucleotide Metabolism - purineChromosomal Location of Human Ortholog: 1q21Cellular Component: cytoplasmMolecular Function: protein binding
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
