The PTBP3 Polyclonal Antibody (PAC061975) is a valuable tool for researchers studying PTBP3, a key regulator of alternative splicing and gene expression. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting PTBP3 in human samples, making it a reliable choice for Western blot and immunohistochemistry applications.PTBP3, also known as Polypyrimidine Tract Binding Protein 3, is involved in various cellular processes, including mRNA splicing and stability, as well as RNA localization. Dysregulation of PTBP3 has been linked to cancer progression, neurological disorders, and viral infections, highlighting its importance in both normal physiology and disease pathogenesis.
By targeting PTBP3 with this antibody, researchers can gain insights into its function and potential as a therapeutic target for various diseases.Overall, the PTBP3 Polyclonal Antibody offers researchers a powerful tool for investigating the role of PTBP3 in cellular processes and disease pathways, ultimately contributing to advancements in biomedical research and drug discovery efforts.
Western Blot. Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate. All lanes: PTBP3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 57, 61, 50 kDa. Observed band size: 60 kDa.
Immunoprecipitating PTBP3 in A549 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO61975 in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000). Lane 2: PACO61975 (5µg) + A549 whole cell lysate (0.5mg). Lane 3: A549 whole cell lysate (20µg).
IHC image of PACO61975 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U).
Synonyms:
Polypyrimidine tract-binding protein 3 (Regulator of differentiation 1) (Rod1), PTBP3, ROD1
UniProt Protein Function:
ROD1: RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U). 6 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: 9q32Molecular Function: RNA bindingBiological Process: anatomical structure morphogenesis; negative regulation of RNA splicing
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene binds RNA and is a regulator of cell differentiation. The encoded protein preferentially binds to poly(G) and poly(U) sequences in vitro. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
