The PTCH1 Antibody (CAB14772) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications.PTCH1 is a transmembrane receptor protein that serves as a negative regulator of the Hedgehog pathway, controlling the activity of downstream signaling molecules. Dysregulation of PTCH1 has been implicated in various cancers, developmental disorders, and degenerative diseases, highlighting its importance in cellular growth and differentiation processes.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PTCH1 Antibody
SKU:
CAB14772
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PTC, BCNS, PTC1, PTCH, NBCCS, PTCH1
Positive Sample:
Rat skeletal muscle
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
161kDa
Observed MW:
160kDa
This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly.
Purification Method
Affinity purification
Gene ID
5727
RRID
AB_2761648
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat skeletal muscle, using PTCH1 Rabbit pAb (CAB14772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of paraffin-embedded mouse embryos using PTCH1 Rabbit pAb (CAB14772) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
