The CD45 Antibody (CAB0372) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to the PTPRC protein, this antibody enables accurate detection and analysis in a variety of cell types, particularly in studies focusing on immunology and cancer research.
This antibody is validated for use in WB, IF/ICC, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CD45 Antibody
SKU:
CAB0372
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
NLDE QQEL VERD DEKQ LMNV EPIH ADIL LETY KRKI ADEG RLFL AEFQ SIPR VFSK FPIK EARK PFNQ NKNR YVDI LPYD YNRV ELSE INGD AGSN YINA SYID GFKE PRKY IAAQ GPRD
Tested Applications:
WBIF/ICCIPELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF/ICC
1:50 - 1:200
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Membrane, Membrane Raft, Single-Pass Type I Membrane Protein.
Calculated MW:
147kDa
Observed MW:
200-250kDa
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Purification Method
Affinity purification
Gene ID
5788
RRID
AB_2757155
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using CD45 Rabbit pAb (CAB0372) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of paraffin-embedded rat spleen using CD45 Rabbit pAb (CAB0372) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CD45 Rabbit pAb (CAB0372). Western blot was performed from the immunoprecipitate using CD45 Rabbit pAb (CAB0372) at a dilution of 1:1000.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CD45 Rabbit pAb (CAB0372). Western blot was performed from the immunoprecipitate using CD45 Rabbit pAb (CAB0372) at a dilution of 1:1000.
