The PUS1 Antibody (CAB8720) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the PUS1 protein, this antibody enables the detection and analysis of PUS1 in various cell types, making it suitable for investigations in RNA biology and molecular biology research.PUS1, a pseudouridine synthase, plays a crucial role in modifying RNA molecules by converting uridine residues to pseudouridine, thereby influencing RNA structure and function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PUS1 Antibody
SKU:
CAB8720
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MLASA1, PUS1
Positive Sample:
U-87MG
Cellular Localization:
Mitochondrion, Nucleus.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
80324
RRID
AB_2771886
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-87MG cells, using PUS1 Rabbit pAb (CAB8720) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
