The PUS10 Antibody (CAB15962) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It specifically binds to the PUS10 protein, allowing for precise detection and analysis in a variety of cell types.PUS10 plays a crucial role in modifying RNA molecules, particularly tRNAs, to ensure their proper function in protein synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PUS10 Antibody
SKU:
CAB15962
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DOBI, Hup10, CCDC139, PUS10
Positive Sample:
Mouse thymus, Mouse spleen, Mouse testis, Rat thymus
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus.
Calculated MW:
60kDa
Observed MW:
60kDa
Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).
Purification Method
Affinity purification
Gene ID
150962
RRID
AB_2763399
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PUS10 Rabbit pAb (CAB15962) at 1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
