PVDF Membrane (0.45?m) 10 Blots

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€180

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PVDF Membrane(0.45μm)

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Figure 1. Fluorescence image overlays of HeLa cells stained using Assay Genie JC-10 Mitochondrial Membrane Potential assay kit acquired before (left) and after (right)the addition of CCCP, a potent mitochondrial decoupling reagent. Images were acquired using a 20X objective with GFP (green JC-10 monomer) and propidium iodide (orange JC-10 aggregate for healthy mitochondria) filter cubes. — Add to Cart

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Figure 2. Change in green/orange fluorescence ratio in cells treated with 1 or 10 μM CCCP, a potent mitochondrial decoupling reagent, for 4 hours. An increase in green/orange fluorescence is indicative of compromised or depolarized mitochondria. Fluorescence intensities were acquired using a plate reader (Ex/Em 490nm/525nm and 540nm/590nm). — Add to Cart

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