The PYCRL Antibody (CAB17763) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in Western blot applications.PYCRL, also known as pyrroline-5-carboxylate reductase-like protein, plays a crucial role in various cellular processes, including the regulation of proline metabolism and cellular redox balance. Its involvement in these pathways makes it a promising target for research in immunology, metabolism, and cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
PYCRL Antibody
SKU:
CAB17763
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PYCRL
Positive Sample:
Mouse liver
Cellular Localization:
Cytosol, Intercellular Bridge, Mitotic Spindle.
Calculated MW:
29kDa
Observed MW:
35kDa
This gene encodes a protein that belongs to the pyrroline-5-carboxylate reductase family of enzymes. Members of this family catalyze the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline. Glutamate and ornithine are precursors in the synthesis of proline. The protein encoded by this gene is a cytoplasmic enzyme involved in the biosynthesis of proline from ornithine.
Purification Method
Affinity purification
Gene ID
65263
RRID
AB_2771892
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse liver, using PYCRL Rabbit pAb (CAB17763) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
