The PYGM Polyclonal Antibody (CAB9392) is a valuable tool for researchers studying the PYGM protein, which plays a key role in muscle metabolism and energy production. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the PYGM protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for research in physiology, metabolism, and muscular disorders.PYGM, also known as glycogen phosphorylase, is essential for the breakdown of glycogen into glucose during periods of energy demand in muscle cells.
Dysregulation of PYGM function is associated with disorders such as McArdle disease, a rare genetic disorder that affects muscle metabolism. Understanding the function and regulation of PYGM is essential for developing targeted therapies for these conditions and exploring its potential role in metabolic diseases. Researchers looking to investigate the role of PYGM in muscle metabolism and related disorders will find the PYGM Polyclonal Antibody (CAB9392) to be a reliable tool for their studies.
Product Name:
PYGM Rabbit Polyclonal Antibody
SKU:
CAB9392
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 743-842 of human PYGM (NP_005600.1).
This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5837
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using PYGM antibody (CAB9392) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
