The QuickStep Mouse Total Testosterone ELISA Kit is specifically designed for the precise measurement of total testosterone levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research experiments.Testosterone is a vital hormone that plays a crucial role in regulating numerous physiological processes, including muscle growth, bone density, and reproductive functions. Imbalances in testosterone levels can lead to various health issues, making it essential to monitor and study testosterone levels in research settings.
With the QuickStep Mouse Total Testosterone ELISA Kit, researchers can easily analyze testosterone levels in mouse models, allowing for a better understanding of testosterone-related mechanisms and potential therapeutic interventions. This kit is a valuable tool for scientists investigating hormonal regulation, reproductive health, and related metabolic disorders in mouse models.
Product Name:
QuickStep Mouse Testosterone ELISA Kit
Product Code:
QSES034
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Mouse
Sensitivity:
0.14 ng/mL
Range:
0.31-20 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Testosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma.
Specificity:
This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Mouse serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse T are added to the micro ELISA plate wells. Mouse T in samples (or standards) competes with a fixed amount of Mouse T on the solid phase supporter for sites on the HRP linked detection antibody specific to Mouse T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Mouse T in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
1.02
1.87
8.67
1.00
2.01
7.85
Standard deviation
0.06
0.11
0.38
0.06
0.10
0.43
CV (%)
5.89
5.63
4.44
5.80
5.16
5.49
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
93-106
99
EDTA plasma(n=8)
84-96
91
Samples were spiked with high concentrations of Mouse T and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
