The QuickStep Rabbit Cortisol ELISA Kit is specially designed for the rapid and reliable detection of cortisol levels in rabbit serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for researchers in the field.Cortisol is a key hormone that plays a critical role in the body's stress response, immune function, metabolism, and more. Abnormal cortisol levels have been linked to various health conditions, including stress-related disorders, metabolic disorders, and inflammatory diseases.
As such, this ELISA kit serves as a valuable tool for studying cortisol levels in rabbits and understanding its physiological implications.With its user-friendly protocol and quick turnaround time, the QuickStep Rabbit Cortisol ELISA Kit is an essential asset for researchers looking to explore the role of cortisol in rabbit physiology and disease pathology. Trust in this kit for robust and precise measurements that push your research forward.
Product Name:
QuickStep Rabbit Cortisol ELISA Kit
Product Code:
QSES056
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rabbit
Sensitivity:
2.87 ng/mL
Range:
6.25-400 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Cortisol
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum and plasma.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Rabbit serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
21.98
49.37
157.20
20.25
47.56
172.37
Standard deviation
1.53
2.52
8.60
1.11
2.69
5.34
CV (%)
6.97
5.10
5.47
5.50
5.66
3.10
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
87-100
94
EDTA plasma(n=8)
88-101
94
Samples were spiked with high concentrations of Rabbit Cortisol and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
