The QuickStep Rat T Testosterone ELISA Kit is a cutting-edge assay designed for the precise measurement of testosterone levels in rat serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable tool for various research studies.Testosterone is a key hormone that plays a crucial role in numerous physiological processes, including promoting muscle growth, bone density, and libido. Imbalances in testosterone levels can have significant implications for reproductive health, metabolism, and overall well-being.
By accurately measuring testosterone levels, researchers can gain valuable insights into hormone regulation and potential implications for various health conditions.Whether investigating the effects of testosterone on animal models or exploring potential therapeutic interventions, the QuickStep Rat T Testosterone ELISA Kit provides researchers with a reliable and efficient means of quantifying testosterone levels. Trust in this advanced ELISA kit to support your research endeavors and advance scientific understanding in the field of endocrinology.
Product Name:
QuickStep Rat Testosterone ELISA Kit
Product Code:
QSES050
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
0.07 ng/mL
Range:
0.16-10 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Testosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma.
Specificity:
This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Rat serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat T are added to the micro ELISA plate wells. Rat T in samples (or standards) competes with a fixed amount of Rat T on the solid phase supporter for sites on the HRP linked detection antibody specific to Rat T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat T in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
0.51
1.16
4.19
0.46
1.22
4.60
Standard deviation
0.03
0.06
0.22
0.02
0.07
0.22
CV (%)
5.82
5.07
5.36
5.37
5.48
4.74
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
91-102
96
EDTA plasma(n=8)
86-102
93
Samples were spiked with high concentrations of Rat T and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
