The RAB24 Antibody (CAB8394) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the Rab24 protein, this antibody allows for precise detection and analysis of Rab24 in various cell types, making it a crucial component for studies in cell biology and molecular biology.Rab24 is a key player in the intracellular vesicle trafficking pathways, controlling the transport of proteins and lipids within cells.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RAB24 Antibody
SKU:
CAB8394
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAB24
Positive Sample:
HT-1080, MCF7, SH-SY5Y, 293T, Mouse brain
Cellular Localization:
Cytoplasm, Lipid-Anchor, Membrane, Cytosol.
Calculated MW:
23kDa
Observed MW:
23kDa
RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking (Olkkonen et al., 1993 [PubMed 8126105]).
Purification Method
Affinity purification
Gene ID
53917
RRID
AB_2771905
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RAB24 Rabbit pAb (CAB8394) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 15s.
Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg RAB24 antibody (CAB8394). Western blot was performed from the immunoprecipitate using RAB24 antibody (CAB8394) at a dilution of 1:1000.
