The Rabbit anti Myc-Tag Polyconal Antibody (CABE009) is a high-quality antibody developed for reliable detection and analysis of target proteins. Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
This antibody is validated for use in WB, IF/ICC, IP applications and has demonstrated reactivity against Species independent samples.
Product Name:
Rabbit anti Myc-Tag Polyconal Antibody
SKU:
CABE009
Size:
100μL, 200μL, 50μL
Reactivity:
Species independent
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIP
Recommended Dilution:
WB
1:2000 - 1:6000
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
Myc, Myc tag, Myc-tag
Positive Sample:
293T-NLK-Myc
Observed MW:
60kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2771925
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein,using Rabbit anti Myc-Tag pAb (CABE009) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of HeLa-Myc-C and HeLa cells using Rabbit anti Myc-Tag pAb (CABE009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of 293T-NLK-Myc cells using 3 μg Myc-Tag antibody (CABE009). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (CABE009) at a dilution of 1:20000.
