Rabbit BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
The Rabbit BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit is a cutting-edge assay designed to quantitatively measure the levels of BMP-2 in diverse biological samples collected from rabbits. BMP-2, a key signaling molecule belonging to the transforming growth factor-beta superfamily, plays an integral role in bone formation, regeneration, and development, as well as in various cellular processes such as proliferation and differentiation. Accurate quantification of BMP-2 is crucial for investigating bone metabolism, regenerative processes, and skeletal development in rabbits.
The Rabbit BMP-2 ELISA Kit provides researchers with a powerful tool to explore the intricate mechanisms underlying bone health, tissue repair, and osteogenesis in rabbit models. With exceptional sensitivity and specificity, this ELISA kit ensures precise and reproducible results. Manufactured under strict quality control measures, this kit guarantees reliable performance and user-friendly protocols, making it an excellent choice for in-depth research studies focused on understanding BMP-2 functions and implications in rabbits.
Product Name:
Rabbit BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit
SKU:
AEES00568
Target:
Rabbit BMP-2 (Bone Morphogenetic Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit BMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit BMP-2. You can calculate the concentration of Rabbit BMP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
90-101
94-105
Average (%)
91
96
99
1:4
Range (%)
90-104
86-99
82-95
Average (%)
96
92
87
1:8
Range (%)
93-106
81-95
88-102
Average (%)
100
87
94
1:16
Range (%)
89-102
83-97
89-100
Average (%)
96
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.52
191.15
927.55
101.5
208.87
978.53
Standard deviation
6.52
7.65
41.18
6.2
11.4
42.47
C V (%)
6.18
4.0
4.44
6.11
5.46
4.34
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit BMP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit BMP-2 in samples. No significant cross-reactivity or interference between Rabbit BMP-2 and analogues was observed.
