Rabbit CD4 (Cluster of Differentiation 4) ELISA Kit
The Rabbit CD4 (Cluster of Differentiation 4) ELISA Kit is a specialized assay designed for the quantitative detection of CD4 levels in various biological samples obtained from rabbits. CD4, a glycoprotein expressed on the surface of T helper cells, is a critical component of the immune system and plays a pivotal role in orchestrating immune responses and coordinating adaptive immunity.
Accurate quantification of CD4 levels is essential for evaluating immune function, monitoring immune responses, and understanding the immune system's dynamics in rabbits. The Rabbit CD4 ELISA Kit offers researchers a reliable and precise tool to measure CD4 concentrations, enabling detailed investigations into immunity, infection, and immunological disorders in rabbits. With outstanding sensitivity and specificity, this ELISA kit ensures dependable and reproducible results. Manufactured under stringent quality control standards, the kit delivers robust performance and user-friendly protocols, making it an excellent choice for research applications focused on elucidating immune responses and immune-related diseases in rabbits.
Product Name:
Rabbit CD4 (Cluster of Differentiation 4) ELISA Kit
SKU:
AEES00567
Target:
Rabbit CD4 (Cluster of Differentiation 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit CD4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit CD4. You can calculate the concentration of Rabbit CD4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-110
89-102
Average (%)
93
101
94
1:4
Range (%)
87-101
95-112
86-97
Average (%)
93
102
92
1:8
Range (%)
88-100
92-107
87-99
Average (%)
94
99
92
1:16
Range (%)
83-94
91-107
84-96
Average (%)
89
97
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
94-111
101
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.23
15.24
49.03
5.54
14.1
44.56
Standard deviation
0.32
0.62
2.07
0.31
0.65
1.82
C V (%)
6.12
4.07
4.22
5.6
4.61
4.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit CD4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit CD4 in samples. No significant cross-reactivity or interference between Rabbit CD4 and analogues was observed.
