The Rabbit ELN (Elastin) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of Elastin levels in various rabbit biological samples.
Elastin is a vital component of the extracellular matrix, providing elasticity and resilience to tissues such as skin, arteries, and lungs. By accurately measuring Elastin levels, researchers can gain insights into the structural integrity and flexibility of tissues, as well as the role of Elastin in maintaining tissue function and health. This ELISA kit offers exceptional sensitivity and specificity, enabling reliable and reproducible results in the assessment of Elastin content. With stringent quality control standards in place, this kit ensures robust performance and accuracy, making it an excellent choice for research focused on understanding tissue integrity and the impact of Elastin in various physiological and pathological conditions.
Product Name:
Rabbit ELN (Elastin) ELISA Kit
SKU:
AEES00571
Target:
Rabbit ELN (Elastin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit ELN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit ELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit ELN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit ELN. You can calculate the concentration of Rabbit ELN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
84-98
94-107
Average (%)
103
91
100
1:4
Range (%)
92-105
83-97
91-102
Average (%)
99
89
96
1:8
Range (%)
98-111
81-94
97-109
Average (%)
104
86
103
1:16
Range (%)
95-108
84-98
89-104
Average (%)
101
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.81
37.98
169.35
20.1
35.85
174.82
Standard deviation
1.17
1.55
5.76
1.09
1.67
5.38
C V (%)
6.22
4.08
3.4
5.42
4.66
3.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit ELN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit ELN in samples. No significant cross-reactivity or interference between Rabbit ELN and analogues was observed.
